Regulatory

Part:BBa_K1484340

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI24, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 258
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1030
    Illegal XhoI site found at 264
    Illegal XhoI site found at 377
    Illegal XhoI site found at 420
    Illegal XhoI site found at 464
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 78
    Illegal BsaI.rc site found at 427
    Illegal BsaI.rc site found at 538


[edit]
Categories
//promoter
Parameters
None